Dopamine receptors in human lymphocytes: Radioligand binding and quantitative RT-PCR assays
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چکیده
منابع مشابه
Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays.
A single tube, fluorogenic probe-based, real-time quantitative reverse transcription-polymerase chain reaction (Q-RT-PCR) assay was developed for detection and quantitation of bovine respiratory syncytial virus (BRSV) using BioRad's iCycler iQ. Real-time Q-RT-PCR was compared with quantitative competitive RT-PCR (QC-RT-PCR) and viral titers. Viral mRNA levels were measured in BRSV-infected bovi...
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We have compared a new enzyme immunoassay (EIA) for estrogen receptors (ER) with our conventional radioligand binding assays (multipoint dextran-coated charcoal assay for cytoplasmic ER and hydroxylapatite exchange assay for nuclear ER). Cytoplasmic ERs were measured in 76 human breast cancer specimens by EIA and by five-point Scatchard analysis. The correlation between the two assays yielded a...
متن کاملQuantitative PCR and RT-PCR in virology.
I n the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relations...
متن کاملAlpha1-adrenoceptor subtypes on rat afferent arterioles assessed by radioligand binding and RT-PCR.
We utilized [3H]prazosin saturation and competition radioligand binding studies to characterize the expression of alpha1-adrenoceptors in preglomerular vessels. mRNA for adrenoceptor subtypes was assayed using RT-PCR. The vessels were isolated using an iron oxide-sieving method. [3H]prazosin bound to a single class of binding sites (Kd 0.087 +/- 0.012 nM, Bmax 326 +/- 56 fmol/mg protein). Phent...
متن کاملa1-Adrenoceptor subtypes on rat afferent arterioles assessed by radioligand binding and RT-PCR
Salomonsson, Max, Melinda Oker, Susan Kim, Hua Zhang, James E. Faber, and William J. Arendshorst. a1-Adrenoceptor subtypes on rat afferent arterioles assessed by radioligand binding and RT-PCR. Am J Physiol Renal Physiol 281: F172–F178, 2001.—We utilized [3H]prazosin saturation and competition radioligand binding studies to characterize the expression of a1-adrenoceptors in preglomerular vessel...
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ژورنال
عنوان ژورنال: Journal of Neuroscience Methods
سال: 2008
ISSN: 0165-0270
DOI: 10.1016/j.jneumeth.2008.07.018